Illumina HiSeq and MiSeq technologies

Various library types are available for Illumina HiSeq and MiSeq sequencing, including:

  • gDNA libraries (shotgun and PCR-free) for the sequencing of whole genomes on the HiSeq X Nextera libraries (Illumina) for the sequencing of small genomes or amplicons
  • ChIP-Seq libraries
  • RNA-Seq
  • Whole genome bisulfite libraries for the identification of methylation sites
  • Reduced genome libraries
    • Exome captures (Agilent SureSelect; Roche Nimblegen SeqCap; Illumina Nextera Exome)
    • Custom panels (Roche Nimblegen SeqCap; Illumina Nextera Capture)
    • Methylation (Roche Nimblegen SeqCapEpi)
  • Custom Amplicons
  • Metagenomics (16S/18S/ITS)

Most library preparation pipelines have been automated to increase robustness and cost efficiency.

Libraries can be sequenced in either single-read of paired-end formats and for read lengths of 50, 100, 150, or 250 bases (up to 300 bases for the MiSeq). On average, 15 M reads can be obtained on the MiSeq and from 150M to over 450M reads can be obtained on a single lane of HiSeq flowcell depending on the sequencing mode selected.

Sequences can be downloaded directly from Nanuq. Sequence reads will be delivered as BAM files for human, rat and mouse and as fastq for the other species.

Download the Technical Application Note on automated size selection of DNA Fragments using SPRI select beads.

More information can be found in the User Guide or by contacting the Client Management Office.